The EpiDirect® MGMT Methylation qPCR Assay represents a new and innovative approach allowing for direct quantification of MGMT promoter methylation by qPCR without prior DNA conversion.
The EpiDirect® technology is based on our unique INA® chemistry and includes an EpiPrimer™ oligonucleotide that specifically binds and primes amplification of methylated target DNA.
The assay is able to quantify MGMT promoter methylation using standard real-time PCR instruments* in just two hours with as little as 0.5 ng of unconverted input DNA.
The assay is compatible with FFPE and fresh-frozen glioblastoma samples and eliminates the need for time-consuming bisulfite conversion.
- Direct qPCR analysis without DNA pretreatment
- Fast results in under 2 hours
- High sensitivity: detects methylation levels as low as 1.5%
- Standard qPCR instrument procedure
- Minimal hands-on time
- 0.5-50 ng input DNA
- CE IVDR certified for clinical use
Link to product details on PentaBase.com – Click here –
Link to IFU – Click here –
